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KMID : 0613820100200121801
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Journal of Life Science
2010 Volume.20 No. 12 p.1801 ~ p.1806
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Isolation, Purification and Characterization of the β-Xylosidase from Klebsiella sp. Sc.
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Lee Yong-Seok
Park In-Hye
Ahn Soon-Cheol
Choi Yong-Lark
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Abstract
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A β-xylosidase encoding gene from Klebsiella sp. Sc was cloned in Escherichia coli. The β-xylosidase gene consisted of an open reading frame of 1,680 nucleotides and encodes 559 amino acids with a deduced molecular weight of 63 kDa. The deduced amino acid sequence of the β-xylosidase from Klebsiella sp. Sc exhibits 90% identities and 95% positives compared to those from Klebsiella oxytoca (KOX), Lactobacillus lactis (LAC, 82%, 90%), Bacillus longum (BLON, 69%, 81%) and Escherichia coli (ECOLI, 47%, 63%). The β-xylosidase was purified by GST-fusion purification system. The pH and temperature optima of the enzyme were 6.6 and 55℃, respectively. The β-xylosidase hydrolyzes xylobiose to xylose.
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KEYWORD
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Klebsiella sp, β-xylosidase, glycosyl hydrolase family 43
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